Insertional mutagenesis using t - dna , transposons and plasmids axe commonly methods to creating a mutant library . here we summarize these strategies as well as the progress in the functional genomes research 本文分別介紹三種方法的原理及其在功能基因組學(xué)研究中的應(yīng)用和研究進(jìn)展。
Exponential phase cultures of the dr rl strain survive exposure to gamma radiation at doses as high as 5 , 000 gy without loss of viability or evidence of dna damage induced mutation . 6000 gy of irradiation will induce approximately 200 dna double - strand breaks in dr chromosomes , but it is still able to reconstruct a functional genome from chromosomal fragments without any mutation 指數(shù)生長(zhǎng)期的r1菌株即使在5kgy劑量的-射線(xiàn)照射后,其生長(zhǎng)能力也未受影響, 6kgy -射線(xiàn)照射后染色體基因組產(chǎn)生約200個(gè)的雙鏈斷裂碎片,但是其基因組dna經(jīng)修復(fù)后沒(méi)有引起任何的突變。